MICROBIOLOGY: Question 8

How to differentiate P. aeruginosa vs P. putida ?

Answers:  Growth at 42 C

Notes: Growth at 42 degrees C is advocated to differentiate species of the fluorescent pseudomonas group as well as to differentiate other nonfermentative bacteria.  Pseudomonas aeruginosa grew abundantly after overnight incubation, whereas 16 of 74 isolates of P. putida (22%) showed slight turbidity in the broth after 24 or 48 h which could not be regarded as an inoculum effect.

SOURCE: ncbi.nlm.nih.gov

MICROBIOLOGY: Question 7

What is the specifications for Autoclave sterilization?

Answers:  121C(250 F) , 15 psi, 15 mins

MICROBIOLOGY: Question 6

What is the biosafety level of Mycobacterium tuberculosis?

Answers:  BSL III

SOURCE:  cdc.com and alyvea.com

MICROBIOLOGY: Question 5

What is the test associated with Scarlet Fever?

Answers:  Dick Test

 

NOTES: 

This is a method of determining susceptibility to scarlet fever by injection into the skin of 0.1 cubic centimetre of scarlet fever toxin. A reddening of the skin in an area over 10 millimetres (0.4 inch) in diameter within about 24 hours indicates a lack of immunity to the disease. Scarlet fever is a bacterial infection caused by Group A streptococcal bacteria, commonly affecting children.

 

SOURCE:  britannica.com

Microbiology: Mnemonic #1

CULTURE PLATES

BBE (Bacteroide-Bile Esculin) - Bacteroides fragilis

CCFA (Cycloserine Cefoxitin Fructose Egg Yolk) - Clostridium difficile

EYA (Egg Yolk Agar) - Clostridium & Fusobacteria (Lecithinase & lipase prod)

LKV (Laked Kanamycin-Vanco) - Bacteroides & Prevotella

Thio - Aerobe, facultative anaerobe

Martin Lewis - Neiserria (CO2 ↑) BCYE (Buffered Charcoal Yeast Extract) - Legionella & Tularemia Regan Lowe + Bordet-Gengou - Bordettella pertussis Rabbit BA/Horse - Haemophilus TCBS - Vibrio LIM Broth (Todd Hewitt) + Colistin & Nalidixic - GBS Human blood tween agar (HBT) - G. vaginalis CIN (Cefsulodin-Irgason Novobiocin) - Y.enterocolitica (Aeromonas & Plesiomonas) XLD (Xylose-lysine deoxycholate) - Salmonella & Shigella HE (Hektoen enteric) - Enteric pathogen (stool) Cystine-tellurite BA - C. diptheriae (black colonies) Loeffler medium - C. diptheriae Tinsdale agar - C. diptheriae

MICROBIOLOGY: Question 4

What is the rapid test for Legionella?

Answer:  Urine Antigen

Source: Dr.T.V.Rao MD

MICROBIOLOGY: Question 3

What parasite is shown?

Answers:  Giardia 

Source: www.atlas-protozoa.com

MICROBIOLOGY: Question 2

Meat cutters/ Butchers are at greatest risk of exposure of what type of bacteria?

Possible answers:  Erysipelothrix spp and Brucella Spp

Sources: http://cttm-kenya.com/docs/brucellosis.pdf     

               https://cmr.asm.org/content/cmr/2/4/354.full.pdf

Microbiology: Mnemonic #1

So this mnemonic only makes sense for Filipino students (sorry).  This is a funny and graphic way of remembering organisms that yield a positive reaction for H2S may it be in the TSI. 

H2S Positive :  Si Ed poop Salmonella.

• Si  :     Citrobacter
• Ed:      Edwardsiella
• Poop:  Proteus
• Salmonella typhi
• H2S (+) is black and a poop is somehow black

MICROBIOLOGY: Question 1

What is the organism that causes/ is associated with Cystic Fibrosis?

Possible answers:  Pseudomonas aeruginosa, Burkholderia cepacia 

Source: http://www.ncbi.nlm.nih.gov/